When moving your microscope, always carry it with both hands (Figure 1).
Grasp the arm with one hand and place the other hand under the base for
2. Turn the revolving nosepiece so that
the lowest power objective lens is "clicked" into position.
3. Place the microscope slide on the
stage and fasten it with the stage clips. You can push down on the
back end of the stage clip to open it.
4. Using the coarse adjustment, lower the
objective lens down as far as it will go without touching the slide!
Note: Look at the slide and lens from the side when doing this (see
5. Look through the eyepiece and adjust
the illuminator (or mirror) and diaphragm (Figure 3) for the greatest
amount of light.
6. Slowly turn the coarse adjustment so
that the objective lens goes up (away from the slide). Continue
until the image comes into focus. Use the fine adjustment, if available,
for fine focusing.
7. Move the microscope slide around so
that the image is in the center of the field of view and readjust the
mirror, illuminator or diaphragm for the clearest image.
8. You should be able to change to the
next objective lenses with only slight focusing adjustment. Use the fine
adjustment, if available. If you cannot focus on your specimen, repeat
steps 4 through 7 with the higher power objective lens in place. DO NOT
ALLOW THE LENS TO TOUCH THE SLIDE!
9. The proper way to use a monocular
microscope is to look through the eyepiece with one eye and keep the
other eye open (this helps avoid eye strain). If you have to close one
eye when looking into the microscope, it's ok. Remember,
everything is upside down and backwards. When you move the slide to the
right, the image goes to the left!
10. Do not touch the glass part of the
lenses with your fingers. Use only special lens paper to clean the
lenses. (read the page on keeping your microscope clean)
11. When finished, raise the tube, click
the low power lens into position and remove the slide.
Remember, microscopes are expensive
scientific instruments. Handle them properly and carefully and they will
last for many years!